Detailed Information [140677]
 

Strain Information
DGRC Number 140677
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E PBac{SAstopDsRed}LL02727 / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E PBac{SAstopDsRed}LL02727 / TM6B, Tb1
Break points/Insertion Site 98F1
Related Genes CG31048
Received Date 25 October 2007
Original Number LL02727
Chromosome 3
Comments No Tb expression but Hu is OK. 26Apr2017 E.O.
Original Source Liqun Luo, Stanford University
Original Comments
Location: ~3R:24689067(-)
Cytological Band: 98F1
Gene Symbol-1: CG31048
CG Number-1: CG31048
FlyBase ID-1: FBgn0051048
Insertion Type-1: CDS
Gene Symbol-2:
CG Number-2:
FlyBase ID-2:
Insertion Type-2:
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
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Library & Clone Information
Strand Minus
Insertion Point 24689067
Chromosome Band 3R
Flanking Sequence 
AAAAGGGATAAAAAGCTATCAAATACGAGAATTATATGCGTTCTAGGGTTTCTACTTACA
GAGTCGGGGAACTTTGAGGCTGTTCTTGTTCACATATATTAATCATCTGTTTTTACGTTC
CACAGGGGCGCCTAACTAGATAACGTAATACGACTCACTATAGGGCGAATTGGAGCTCCA
CCGCGGTGGCGGCCGCTCTAGAACTAGCTAGttctagtgttcccacaatggttaattcga
TCTTCTTAATTGAGTCCGACTGAATGATCTTGCCAACGCGCATGACCACGGCCACCAGGT
GAAGTTCCTCATTTAGATCAGCAGCACCTGAAAATGTGAAATGGTTTAGATCATACAACA
TATTGATATGATAACTCCCATACCCAAGTCAGTGAACACCGTGCAGTTGCTGTGCAACCT
TTCAATGTAATTGGAGAAACCATCCTTGGAGATTTTGACCAGAAACCGTTCGGACAGCGG
TCGCATGTGACTGCCATCATAGAGGAAGAAGTAGACCTCCGCATCATCGCCACCGATCCG
ATGGCCAAAATCGCGCATGCAAAAGTACAGGTGGTGGGTGAGTATCTTGCGCTGCGTCTT
CCGCCTCAAAGTTCCCCGACTCTGTGGGTTGTtaaccctagaaagatagtctgcgtaaaa
TTGACGCATGCATTCTTGAAATATTGCTCTCTCTTTCTAAATAGCGCGAATCCGTCGCTG
TGCATTTAAGACATCTCAGTCGCCGAATATAGTCTTTCTTTTAACAATGGTAAAATGGAA
ATAAAAACATTAATAATTAGAAATAATTATATAATTTAGAAGAG
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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