Detailed Information [140779]
 

Strain Information
DGRC Number 140779
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL03667 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL03667 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 86D8
Related Genes RpL3 (CG4863), RpL3 (CG4863)
Received Date 25 October 2007
Original Number LL03667
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:7047729(+)
Cytological Band: 86D8
Gene Symbol-1: RpL3
CG Number-1: CG4863
FlyBase ID-1: FBgn0020910
Insertion Type-1: 5' UTR
Gene Symbol-2: RpL3
CG Number-2: CG4863
FlyBase ID-2: FBgn0020910
Insertion Type-2: Intron.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Plus
Insertion Point 7047729
Chromosome Band 3R
Flanking Sequence 
tttacgcagactatctttctagggttaaATAATTGATTAGTTGGGAAACAGTAGCGTTTT
AATCAATCTAAATGTAACTAATACAAACAGCGTAATTTTAATGCATAATTTGCACTTGCA
ACATCCACGTGTTCACCTAACCTCAAAGTGCAGAAACGCATTGTGCTAATGGATTTTCTC
CATTCATTTTGCAGTCTCATCGTAAGTTCTCGGCACCCCGCCATGGCTCCATGGCCTTCT
ACCCCAAGAAGCGCTCAGCTCGCCATCGCGGCAAGGTTAAGGCCTTCCCCAAGGATGACG
CCAGCAAGCCAGTCCATCTGACCTGCTTCATCGGCTACAAGGCCGGTATGACCCACATTG
TGCGCGAGGCCGATCGTCCTGGCTCCAGTAAGTACCAGCATCCACCTCTATCAACTAACA
ATAACTACTTTCCATGATGAGTATACTAGAAGATTTATGGTTAGAACACATATGATTAAC
CGTGATTATCTCAACTAGGGCGGACTGACCAAGCCCACTCAGTAGATGATTTCTCTTAGG
GAATACCATTACTCGGTATTAATGGCTTTTCTTTCTCTTGCAGAGATCAACAAGAAGGAG
GTGGTcgaattaaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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