Detailed Information [140817]
 

Strain Information
DGRC Number 140817
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL03812 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL03812 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 66B8
Related Genes CG7504, mus301 (CG7972)
Received Date 25 October 2007
Original Number LL03812
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:8130587(+)
Cytological Band: 66B8
Gene Symbol-1: CG7504
CG Number-1: CG7504
FlyBase ID-1: FBgn0035842
Insertion Type-1: Putative Promoter
Gene Symbol-2: mus301
CG Number-2: CG7972
FlyBase ID-2: FBgn0002899
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Plus
Insertion Point 8130587
Chromosome Band 3L
Flanking Sequence 
tctagggttaaACTAGCAAAAAGACGCACTTTTTGGTCAGCGGTAAACAGTAAAAGGCAG
TGCTGTTAAACGAATTAGGGGAGTGCGACGTTAAAGTACATTTAGGCGAGTTGTAATTAT
TTGTCGGACCC
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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