Detailed Information [140871]
 

Strain Information
DGRC Number 140871
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL04292 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL04292 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 64E7
Related Genes mule (CG5505), CG10575
Received Date 25 October 2007
Original Number LL04292
Chromosome 3
Comments No Tb expression but Hu is OK. 21Aug2014 M.W.
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:5762087(-)
Cytological Band: 64E7
Gene Symbol-1: mule
CG Number-1: CG5505
FlyBase ID-1: FBgn0035633
Insertion Type-1: Putative Promoter
Gene Symbol-2: CG10575
CG Number-2: CG10575
FlyBase ID-2: FBgn0035632
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Minus
Insertion Point 5762087
Chromosome Band 3L
Flanking Sequence 
tttacgcagactatctttctagggttaaTAAGACATTTGTTACCTTGAATATGTAAGCAA
TATCTTATTGAAAGGCTTGCAGCGACATTTTTTATTTATGCCTACTATTCAAGTTATAAA
TTTAATTTTTATAACGGTATTTTTACACCTTATCAGCACATATcgaattaaccattgtgg
gaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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