Detailed Information [140872]
 

Strain Information
DGRC Number 140872
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL04294 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL04294 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 92F1
Related Genes CG5180, att-ORFA (CG4241)
Received Date 25 October 2007
Original Number LL04294
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:16383667(+)
Cytological Band: 92F1
Gene Symbol-1: CG5180
CG Number-1: CG5180
FlyBase ID-1: FBgn0043457
Insertion Type-1: 5' UTR
Gene Symbol-2: att-ORFA
CG Number-2: CG4241
FlyBase ID-2: FBgn0067783
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Library & Clone Information
Strand Plus
Insertion Point 16383667
Chromosome Band 3R
Flanking Sequence 
tttacgcagactatctGTTCTAGGGTTAAACAAAATATTAAACATAAATAAACATTAAAA
TATATTATTAAACAAAATATATTCTTGACTTGCAATAGATATGCCGTATCACTCATAAAT
GCAATACAAAGGGTAAATCATTATTTCCTGGCTTAAAATTGTAATGAAACGGATAATGAA
TCACATAGCTATTCAGTTTTAGAGAATTTGTATGCACTTTGTCATATTTAACGTTAAAGT
cgaattaaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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