Detailed Information [140879]
 

Strain Information
DGRC Number 140879
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL04466 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL04466 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 90F10
Related Genes 14-3-3epsilon (CG31196), CG7156
Received Date 25 October 2007
Original Number LL04466
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:14068306(-)
Cytological Band: 90F10
Gene Symbol-1: 14-3-3epsilon
CG Number-1: CG31196
FlyBase ID-1: FBgn0020238
Insertion Type-1: 5' UTR
Gene Symbol-2: CG7156
CG Number-2: CG7156
FlyBase ID-2: FBgn0038588
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Library & Clone Information
Strand Minus
Insertion Point 14068306
Chromosome Band 3R
Flanking Sequence 
tttacgcagactatctttctagggttaaAATGGTATTTCTATCTGGATATTACTTGTGAA
AATCCGTTTGTGTGAAGCTTTTATTCGGAAGTTTTAAAATGTTATAGTAAATTGACACAT
TTAAAAAAGCTATAGCTATAAATATGTTGTATTAAATGAACACATGAAGCAATGTCGTTA
GCAGGGACTTCCACCTTTTCTGTGCAATTCAATTTGTCATTAGTCATGACATAACCCACA
TGCACTGCAGACTATcgaattaaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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