Detailed Information [140932]
 

Strain Information
DGRC Number 140932
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL05493 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL05493 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 72D6
Related Genes CG5222
Received Date 25 October 2007
Original Number LL05493
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:16099567(+)
Cytological Band: 72D6
Gene Symbol-1: CG5222
CG Number-1: CG5222
FlyBase ID-1: FBgn0036570
Insertion Type-1: Putative Promoter
Gene Symbol-2:
CG Number-2:
FlyBase ID-2:
Insertion Type-2:
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Plus
Insertion Point 16099567
Chromosome Band 3L
Flanking Sequence 
tttacgcagactatctttctagggTTAAGAAAACCAGGAACACAGGGCCACGATCCTCTA
AACGTTCCGCTGAAAATATGCCAACAGTTACTAAGAAAATTTGCTCTAAGTGGAACGACT
TCTTGTAATTCCTTAAAAATTATATATCATATATATAAATATATATTTGAATTAATTTCA
ATTAGAACTTATTTATAAAGCGAAGAATATTATCAGTGCTAGATTATCTCCAATTAAGAT
TTCCAGTTACGTGAATGAATTAAGCTTATCTTTATTAAAAAAATAATTTAATTATAATTG
TTCAATACGTTTTATTAAAATCCGCTTCGTTCTAGTATTTGTGTATATGAGTGTGAGGGC
CATAATGTTCACATTCAAACTGAATACATTGCCTTGCCTTGCACTAATGCTAGAAAAATA
CAAGTTTTATGATCCACTAAATATTGTTAGTTAAAGTTGGGGTAATTTGGGATTCACTAA
TCGCCGATTGGGCAACATGTTGGACATGCTTAGCTCCTTGTGCCCCAATCGGTTTGAATA
CTCGTGTATAGATTAGAGTTGGTTATAGTTAAGTGACGCCTGGTCGCGCTTTTATCTTGA
GAGAAATTGTAATATTAGTAGCCGACTACGTGTATATAACCTATTCTCGCCGCTTAGGTG
CTGGCAGGAGTGCCTCCGACACCCTCCTTCTTGGCCACCTTCTCATCGGAGCCGGTCTCA
TCGAATTAAACAATTGGGGGAACACCTAGAACTAGCTAGTTCTAGAAGCGG
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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