Detailed Information [141194]
 

Strain Information
DGRC Number 141194
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL04170 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL04170 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 85F4
Related Genes Art4 (CG5358), CG33975
Received Date 9 November 2007
Original Number LL04170
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:5811645(+)
Cytological Band: 85F4
Gene Symbol-1: Art4
CG Number-1: CG5358
FlyBase ID-1: FBgn0037770
Insertion Type-1: 5' UTR
Gene Symbol-2: CG33975
CG Number-2: CG33975
FlyBase ID-2: FBgn0053975
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Stock Request

Library & Clone Information
Strand Plus
Insertion Point 5811645
Chromosome Band 3R
Flanking Sequence
tttgcgcagactatctttctagggttaaTTGGCGCTGGGAACTATATAGTGCGCTGCTTT
TCAACTCGGACGATTACTCAAAATcgaattaaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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