Detailed Information [141226]
 

Strain Information
DGRC Number 141226
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL04311 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL04311 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 85F8
Related Genes mtTFB2 (CG3910)
Received Date 9 November 2007
Original Number LL04311
Chromosome 3
Comments No Tb expression but Hu is OK. 06Jun2014 M.W.
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:5873010(-)
Cytological Band: 85F8
Gene Symbol-1: mtTFB2
CG Number-1: CG3910
FlyBase ID-1: FBgn0037778
Insertion Type-1: 3' UTR
Gene Symbol-2:
CG Number-2:
FlyBase ID-2:
Insertion Type-2:
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Library & Clone Information
Strand Minus
Insertion Point 5873010
Chromosome Band 3R
Flanking Sequence
tttacgcagactatctttctagggttaaAACTAATCTATCATGATTACCTACCAGTTCTT
GATACGTAAGATATTTTTTATTTTTCTTGGGACCCAAACCGCTATTATGTACAACTAGGA
ACTGGCTTTCCTGCGCCTCTTGACGGGAGTGGTGGAGGGAACAGGGCTTTCTTCTTcgaa
ttaaccgatatgggaacact
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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