Detailed Information [141282]
 

Strain Information
DGRC Number 141282
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL04528 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL04528 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 67B8
Related Genes CG3437, phol (CG3445)
Received Date 9 November 2007
Original Number LL04528
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:9449605(+)
Cytological Band: 67B8
Gene Symbol-1: CG3437
CG Number-1: CG3437
FlyBase ID-1: FBgn0035998
Insertion Type-1: CDS
Gene Symbol-2: phol
CG Number-2: CG3445
FlyBase ID-2: FBgn0035997
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Library & Clone Information
Strand Plus
Insertion Point 9449605
Chromosome Band 3L
Flanking Sequence 
tttacgcagactatctttctagggttaaAATCAGTCTTATTAAATTGCACCGCCTTCCTA
TCCGCTGTTAAGGATATATTATAATGCCGACATTGGTCCTGCAATCCTGTTGGCCCATcg
aattaaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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