Detailed Information [141289]
 

Strain Information
DGRC Number 141289
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL04561 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL04561 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 65F6
Related Genes CTCF (CG8591)
Received Date 9 November 2007
Original Number LL04561
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:7349744(+)
Cytological Band: 65F6
Gene Symbol-1: CTCF
CG Number-1: CG8591
FlyBase ID-1: FBgn0035769
Insertion Type-1: 5' UTR
Gene Symbol-2:
CG Number-2:
FlyBase ID-2:
Insertion Type-2:
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-06-11
Research papers using this strain
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Library & Clone Information
Strand Plus
Insertion Point 7349744
Chromosome Band 3L
Flanking Sequence 
tttacgcagactatctttctagggttaaATCTACAATTTAATTTGCGTCCACATGAAACA
GCTGCCCATTTCTAATAAAAGATGGCGACATGTAGCATCAGTACCGCCAGCTATcgaatt
aaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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