Detailed Information [141427]
 

Strain Information
DGRC Number 141427
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL05041 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL05041 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 84D3
Related Genes Gld (CG1152), Pbprp4 (CG1176)
Received Date 9 November 2007
Original Number LL05041
Chromosome 3
Comments No Tb[1] expression but Hu is OK. 1Mar2018 T.O.
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:3077786(-)
Cytological Band: 84D3
Gene Symbol-1: Gld
CG Number-1: CG1152
FlyBase ID-1: FBgn0001112
Insertion Type-1: 3' UTR
Gene Symbol-2: Pbprp4
CG Number-2: CG1176
FlyBase ID-2: FBgn0011282
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Library & Clone Information
Strand Minus
Insertion Point 3077786
Chromosome Band 3R
Flanking Sequence 
tttacgcagactatctttctagggTTAACTACCAAACGATCTCATGAATTTGAAAACATT
TATCGCAAAATAAGCGATAATTGCTATATATATAGAGGCGACAAAATCTGCTTACCAGTC
AGTTTTTCGAAttaaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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