Detailed Information [141478]
 

Strain Information
DGRC Number 141478
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL03704 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL03704 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 85D11
Related Genes CG33936, CG33936
Received Date 19 November 2007
Original Number LL03704
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:5167783(-)
Cytological Band: 85D11
Gene Symbol-1: CG33936
CG Number-1: CG33936
FlyBase ID-1: FBgn0053936
Insertion Type-1: 5' UTR
Gene Symbol-2: CG33936
CG Number-2: CG33936
FlyBase ID-2: FBgn0053936
Insertion Type-2: Intron.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Stock Request

Library & Clone Information
Strand Minus
Insertion Point 5167783
Chromosome Band 3R
Flanking Sequence
tttacgcaggctatctttctagggttaaTCGCGGAGCGCACTAACACGACCGAGAGAGAG
GGAGGGGAGCGCGGAAAAGTGATTACTTTATTATCTAGTTTAGTTAGCAAGGCGCTGACT
GGGCTGACGATGACAGTTGTGGGTAATTGAAGCCAAAAACCAACGTGGATCTGATCCTCC
AAACGCGAAGTGAAGTCTCCAAGCACTCTAACAGAAATGAGCGGACCTCGCAACGCTTGA
TGACTGCGACTGGCGAGCTAACAGAGCTCTGTTGGTGGCAATTTGGTGATGAGAGCGTGA
GTCTGTCACTCAACTTCTCCGGTTGAAAGCAGTTAACAATACCAGGAGTATGTAGGATTG
AAATGATcgaattaaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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