Detailed Information [141509]
 

Strain Information
DGRC Number 141509
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL05139 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL05139 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 64E5
Related Genes DnaJ-1 (CG10578), Ubp64E (CG5486)
Received Date 19 November 2007
Original Number LL05139
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:5745563(-)
Cytological Band: 64E5
Gene Symbol-1: DnaJ-1
CG Number-1: CG10578
FlyBase ID-1: FBgn0015657
Insertion Type-1: Putative Promoter
Gene Symbol-2: Ubp64E
CG Number-2: CG5486
FlyBase ID-2: FBgn0016756
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Library & Clone Information
Strand Minus
Insertion Point 5745563
Chromosome Band 3L
Flanking Sequence 
tttacgcagactatctttctagggTTAAGGAGTACTTTCCGCATTTCGCCATCACAGTCA
CGCTTTCCTTGGCATTTGCAATCAAATAAGCGCTAATAATAATCGTAAAAGCATAAGAAG
CATATAAAGAAGAGTCACCGCCAAAAGCATGCACAAATATATATAAATGGGGAGCGATTT
AAAAACAGTGCACTGTGTTTAAAACATCGAAttaaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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