Detailed Information [141529]
 

Strain Information
DGRC Number 141529
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E PBac{SAstopDsRed}LL05237 / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E PBac{SAstopDsRed}LL05237 / TM6B, Tb1
Break points/Insertion Site 100A1
Related Genes PH4alphaPV (CG31015), CG31013
Received Date 19 November 2007
Original Number LL05237
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:26471931(+)
Cytological Band: 100A1
Gene Symbol-1: PH4alphaPV
CG Number-1: CG31015
FlyBase ID-1: FBgn0051015
Insertion Type-1: 3' UTR
Gene Symbol-2: CG31013
CG Number-2: CG31013
FlyBase ID-2: FBgn0051013
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Library & Clone Information
Strand Plus
Insertion Point 26471931
Chromosome Band 3R
Flanking Sequence 
tttacgcagactatctttctagggTTAAACGATATTTAGCAGGAATTTCCATTTCTTGAT
TTTGATGAACGACTCTGGGGAAAACACCTATTCCTCTTCGGAATGTCGCGACTTGATTTG
CAATTGTTCTTATCGCTGGGAATGTTTAATTGACAGCATTATCGAAttaaccattgtggg
aacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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