Detailed Information [141580]
 

Strain Information
DGRC Number 141580
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL05375 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL05375 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 83B6
Related Genes Atu (CG1433), Rga (CG2161)
Received Date 19 November 2007
Original Number LL05375
Chromosome 3
Comments No Tb expression but Hu is OK. 07Apr2022 E.O.
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:1439161(+)
Cytological Band: 83B6
Gene Symbol-1: Atu
CG Number-1: CG1433
FlyBase ID-1: FBgn0019637
Insertion Type-1: 5' UTR
Gene Symbol-2: Rga
CG Number-2: CG2161
FlyBase ID-2: FBgn0017550
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2022-04-07
Research papers using this strain
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Library & Clone Information
Strand Plus
Insertion Point 1439161
Chromosome Band 3R
Flanking Sequence
tttacgcagactatctttctagggTTAAACAAAATTTACTTGGCATTATGGGCAGCCAAA
ACTCGGACGACGACAGTGGTGAGTGGCTAATGGCTAAGTCCAGGCCTGAGTAACTAATAT
TTACACTGTTCACATATGTATGTAGGTTCATCCGGTTCGAAttaaccattgtgggaacac
tagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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