Detailed Information [141609]
 

Strain Information
DGRC Number 141609
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL05514 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL05514 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 64C7
Related Genes Aats-ala-m (CG4633), CG10671
Received Date 19 November 2007
Original Number LL05514
Chromosome 3
Comments No Tb expression but Hu is OK. 31Jan2013 Y.Y.
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:5139937(+)
Cytological Band: 64C7
Gene Symbol-1: Aats-ala-m
CG Number-1: CG4633
FlyBase ID-1: FBgn0028962
Insertion Type-1: Intron
Gene Symbol-2: CG10671
CG Number-2: CG10671
FlyBase ID-2: FBgn0035586
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Plus
Insertion Point 5139937
Chromosome Band 3L
Flanking Sequence
tttacgcagactatctttctagggTTAAGTACTCGTGCTTTGCAGATTTCCCGCCTCGCG
AATACTGCCCTTTGGCTGCGCCGACAATTTTTGGGAAATGGGCGCTACAGGGCCGTGTGG
TCCCTGCACCGAAATTCACATAGATCATCGCCCGGATCTGGGCAGCGTGGAGCAGCGAGC
CAAGTTGGTGAACGCAGGTCGGTCCGATCTCACAGAGCTGTGGAATCTTGTCTTTATACA
GTACAATCGGTGAGGATTTGAATTCAAGTTGAATGTATTCCTAGCCTAAGATTCTTTATG
CATTGATAGGCATGCCGATGGCAGCATCTCTCAATTGCCCGCTCATCATGTGGACACCGG
CATGGGTTTTGAACGGCTAACGGCTGTGCTTCAGAATAAGTCTTCTAACTATGATACGGA
CTTGTTCACGCCGATATTTGATGGCATCCAGCAGGCGGCCAAGACTCCGGTTTACAGCGG
CAGCTTTCCGGATGGTGGAAATGCAGCAGTACTCGAAttaaccattgtgggaacactaga
ac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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