Detailed Information [141622]
 

Strain Information
DGRC Number 141622
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL05538 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL05538 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 78C8
Related Genes CG32436, CG12972
Received Date 19 November 2007
Original Number LL05538
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:21339938(+)
Cytological Band: 78C8
Gene Symbol-1: CG32436
CG Number-1: CG32436
FlyBase ID-1: FBgn0052436
Insertion Type-1: Intron
Gene Symbol-2: CG12972
CG Number-2: CG12972
FlyBase ID-2: FBgn0037076
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Plus
Insertion Point 21339938
Chromosome Band 3L
Flanking Sequence 
tttacgcagactatctttctagggTTAACAGACATATCTGGTCCCTTGTCCCTTTGCGAC
TACTTACCCCTTTGGTGTTGTTGTTGTTGTTGCGCCGCAACGTTGTTGTTGGCAGGGCGG
CAATAGCAGCCATTTCAATAGTTGTGTTAAGTCAATTGCAACTAGGTTTCTGCTCTTTCC
GTTCTCCTTCTTTTCCTTCTCCACTTCTTCTTTTCCGATAACTGCGCAGCAGCTTCTTGC
GCTCTTACGGCTGTAGTCGTTCCGCTCTCGCGCCACTTAGATCATGCGTCAGAGGGAGAT
AGAGATGTGTTCGTGCGAGACTCCTTTTTGAAGCTGTTACAGAGAAAACGTAATAAAACA
GAAATAAAAGCAATATTAATACTGTTGATGCAAATGGCTAAGCGTTTGTTAGTCACTTTT
TACGAAAGTTGGCACGGCACGTTCTCAAATTCGCTTTGCCTCTGTATTAGCTTTTGTTGT
TGCGCCTCTGACATTTTTACAATTACTGAGTAATAACACACTTGTGTGCCCTGTTTCTGT
TTCTTGGTTCCGCTCTTCGTTTCTCTGCGCCTGTGTGTGTGTGCGTTCAGTGCGCGCTCC
GTATGAAATAAAAACGGTTCACTATGCTCAATCCCTTTTGCATGGTGTTTGCTGTTGTAG
CAACGCAGCTGTTACTTTTGTACAGTGCAGGTCAAGTTACTAGCGTACTTCACTAGAAAG
GGTATTAAACCACTAGACAATGCTTAGATGATTTGATTGTAGGAAGAGATTAGAGCTGAT
TCTGGCAAACCAGGGAAGCAAGCAGAATTATTTTTG
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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