Detailed Information [141627]
 

Strain Information
DGRC Number 141627
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL05549 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL05549 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 89B17
Related Genes Pak3 (CG14895)
Received Date 19 November 2007
Original Number LL05549
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:12278397(-)
Cytological Band: 89B17
Gene Symbol-1: Pak3
CG Number-1: CG14895
FlyBase ID-1: FBgn0044826
Insertion Type-1: CDS
Gene Symbol-2:
CG Number-2:
FlyBase ID-2:
Insertion Type-2:
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Minus
Insertion Point 12278397
Chromosome Band 3R
Flanking Sequence 
tttacgcagactatctttctagggTTAAGAAGAAAGAGAACGAGGTCTTCAAGCCCTTCA
TCACCGAAGATGTGATCCACGAGGAGTCCAAGGAGATAGAAAACTATGTCAACTACAAAA
ACAAGCACAAGTCTCAGGATCCCGAAAAGTCCGACGACGATGGCAGTTCGAAttaaccat
tgtgggaacactagaacTAGCTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAATT
CGCCCTATAGTGAGTCGTATTACGTTATCTAGTTAGGCGCGCCTGTGGGACGGAAGAACA
GATGAATTAGATATCTATAACAAGAAAATATATATATAATAAGTTATCACGTAAGTAGAA
CATGAAATAACAATATAATTATCGTATGAGTTAAATCTTAAAAGTCACGTAAAAGATAAT
CATGCGTCATTTTGACAAGGCGGTCGAAGCATATATAGAGTTTGTGTGTCAAGGGGGGGG
GAAAGGGGTAAGAGCTCGCCGTCTTGCCACCCGCGTCTCGGGGACTTTTCCTCATTCGAA
ttaaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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