Detailed Information [141632]
 

Strain Information
DGRC Number 141632
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL05556 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL05556 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 78E5
Related Genes Rpb8 (CG11246), CG11247
Received Date 19 November 2007
Original Number LL05556
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:21722396(+)
Cytological Band: 78E5
Gene Symbol-1: Rpb8
CG Number-1: CG11246
FlyBase ID-1: FBgn0037121
Insertion Type-1: CDS
Gene Symbol-2: CG11247
CG Number-2: CG11247
FlyBase ID-2: FBgn0037120
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
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Library & Clone Information
Strand Plus
Insertion Point 21722396
Chromosome Band 3L
Flanking Sequence 
tttacgcagactatctttctagggTTAAGATGGACCTCATCCTGGACATCAACTCGTGGC
TATACCCCATGGAGCTGGGCGACAAGTTCCGCCTGGTTTTGGCCACCACCCTGCGCGAGG
ATGGTTGCCCGGACAGCGGGGAGTACAATCCAATGGAGCACGAGGGAACGCGGGCGGACA
GCTTTGAGTACGTGATGTACGGCAAGATCTACAGGATCGAAttaaccattgtgggaacac
tagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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