Detailed Information [141750]
 

Strain Information
DGRC Number 141750
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL06054 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL06054 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 61C1
Related Genes CG32343, Ptpmeg (CG1228)
Received Date 19 November 2007
Original Number LL06054
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:327710(-)
Cytological Band: 61C1
Gene Symbol-1: CG32343
CG Number-1: CG32343
FlyBase ID-1: FBgn0052343
Insertion Type-1: Intron
Gene Symbol-2: Ptpmeg
CG Number-2: CG1228
FlyBase ID-2: FBgn0035133
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Minus
Insertion Point 327710
Chromosome Band 3L
Flanking Sequence 
tttacgcagactatctttctagggTTAATAAACTTATTGAAGTAATGCCAATTATAGGCC
TTCACTCGTCCCTGAAAGTGTTCTTCTGCTAAAATGCACAGTCTCGGCGCCAGCAAAGTC
GTTGCTACTCTCCGAGCGGATGGTCACGTCTACCCCATGGAGAACTCCAACCTGATGCAC
TACGAGATCGTAAGACTCGCGGTACCTTGCTTGTAATTCACACTAACCGAGGCGACTCCT
TCATAGCGCACCGAAGGGGATGTCCAGCAAAAGAAGGGGATCCTGATGGGTCCGGCCACA
TGCGTTGACCTGGGCAAACAGCTGCTCCAGTGCGCCAGAGACAGCGATGTAGCTGGGGTC
AAGGCGGCGCTAGCGCATGGAGCTCCCTTCGCTTCCGACTGGCTAGGTATGTCGGCACTT
CACTTTGCCGCCATGAACAATCAGCTGGAGATTTGCGAAATCCTCCTGCAGGGGGGCATT
AACATGGATGGCAAGACCAAGGTAGACCGAACGCCGCTCCACCTAGCCTGCTACTACGGT
CACGAGCGGATAGTCAGCTTGCTGCTAGCGCTAAAGTGCAGCGTCAACTCCCGCGACATG
GTAAGAATATATAAATAAATGACTGTTTAAAATGACTAACTAACATTCCTGTCAGCTTCG
CATGACCCCGCTGCACTGGGCGGTCGAAttaaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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