Detailed Information [141787]
 

Strain Information
DGRC Number 141787
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL06364 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL06364 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 95D10
Related Genes Rox8 (CG5422), CG5986
Received Date 19 November 2007
Original Number LL06364
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:19873711(-)
Cytological Band: 95D10
Gene Symbol-1: Rox8
CG Number-1: CG5422
FlyBase ID-1: FBgn0005649
Insertion Type-1: 5' UTR
Gene Symbol-2: CG5986
CG Number-2: CG5986
FlyBase ID-2: FBgn0043455
Insertion Type-2: 5' UTR.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
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Library & Clone Information
Strand Minus
Insertion Point 19873711
Chromosome Band 3R
Flanking Sequence 
tttacgcagactatctttctagggTTAATTAGTTCGCATTTTAGACGACGAAGGTAAAAT
TTTAGCGACGCGTTCAACGTACATTTGCAGCCCCTAACAACTTTGAAAGTTTCGCAAAAT
TAATACAAACCGGTAGCGGCTAAACTTGACCGTTAAATGAAGTGTGCGAACAGAGTGCAG
AGCAAGTAAAATGCGGTAAATTTACAAACGCAGCCACGTCACAGAGAGCGCATTAAAGAA
AAAAGAAGAGAGTGCGAATTTTGACAGCGAAATTCGAAttaaccattgtgggaacactag
aac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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