Detailed Information [141798]
 

Strain Information
DGRC Number 141798
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL06392 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL06392 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 62B4
Related Genes alpha-Spec (CG1977), dlt (CG32315)
Received Date 19 November 2007
Original Number LL06392
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:1794517(+)
Cytological Band: 62B4
Gene Symbol-1: alpha-Spec
CG Number-1: CG1977
FlyBase ID-1: FBgn0003470
Insertion Type-1: Intron
Gene Symbol-2: dlt
CG Number-2: CG32315
FlyBase ID-2: FBgn0024510
Insertion Type-2: 5' UTR.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Plus
Insertion Point 1794517
Chromosome Band 3L
Flanking Sequence 
tttacgcagactatctttctagggTTAATTGAAGTGTCTATCAGAAAATAGCAGAGGATG
ACGTAATGCGTACGCTTTGGAATTATGAGCAATCAATGAAGAGGCACAAAAAATCAACTG
GTACGCAAGACTACTACGAACACGGAATCGCCTGGCAAGGAAACAAAAATATCCTATGCA
AACACAGGCCTTCAAGAAAGTCAAGGACACTTGCCCACCGAAAACAGGTTTTGGCATATG
TAAGGCAAGCACTTTGCTTTTTTTTTATTCGTACTTAAATATCTTGCAAACACAAACAAG
CAAAGAGAGGGAGAGTGTGCTCTCCCGCTTTCTCTCATCTGAAGTTGCCGCACCACCACC
ACCACCCGATGACGTACACACACACAGCGTTCTCTACTCCTGCGTAACCGCACACACACA
TGCACAACTGGAGCACCAGATACTTTTTCTTAAGTTGCAATTGGAGTCAAGAATTTTGAA
AGAAATTCAAAATTTCTATTACTAATCAAGCAGTGACATTGCACAGCATAGCTTTTCAGT
TATGGCCAAGACTTTTCTCTCACTTTAGCCCCGTTAATTGGATTTTTGTGTTGCCTTCGA
Attaaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

CLOSE