Detailed Information [141899]
 

Strain Information
DGRC Number 141899
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL06736 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL06736 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 64A4
Related Genes Gr64e (CG32258), Gr64d (CG14987)
Received Date 11 December 2008
Original Number LL06736
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:4035108(+)
Cytological Band: 64A4
Gene Symbol-1: Gr64e
CG Number-1: CG32258
FlyBase ID-1: FBgn0045476
Insertion Type-1: Intron
Gene Symbol-2: Gr64d
CG Number-2: CG14987
FlyBase ID-2: FBgn0035486
Insertion Type-2: 3' UTR
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Library & Clone Information
Strand Plus
Insertion Point 4035108
Chromosome Band 3L
Flanking Sequence 
tttacgcagactatctttctagggTTAATATTTCATTATATCTATATGTTAATGTATGAA
TTTGTACCTATATTTTTCTAGGGCCAAGCCGTCTATATGGCATGCCCTGTACTTCTGGTT
CTCGCTGGTCTACCTACTTGGTCGCACCCTCATCCTGTCCCTGTACAGCTCCAGTATAAA
TGATGAGTCCAAGCGACCACTGGTAATCTTTCGAAttaaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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