Detailed Information [141946]
 

Strain Information
DGRC Number 141946
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL07012 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL07012 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 88C3
Related Genes Pde6 (CG8279), CG8461 (CG8461)
Received Date 11 December 2008
Original Number LL07012
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:10340061(-)
Cytological Band: 88C3
Gene Symbol-1: Pde6
CG Number-1: CG8279
FlyBase ID-1: FBgn0038237
Insertion Type-1: 3' UTR
Gene Symbol-2: CG8461
CG Number-2: CG8461
FlyBase ID-2: FBgn0038235
Insertion Type-2: Putative Promoter
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Minus
Insertion Point 10340061
Chromosome Band 3R
Flanking Sequence 
CCTTAGATGCATGCGTCATTTTACGCACACTATCCGTTCTAGGGTTAATATGAATAATCC
GGTCCCACACTCTCCTAGTTGTTGCAATCAAGTTTACTTTAAATGTGTGTACGTGTACAA
AGCAAACTACTTATATACTATAGAGCATAAATATAAACTAATTATACATATACGGAACCT
ATATAAAATATTTACATTTGACTAATGTATCTATAACTGAATTGCTTAGAATTAACGTGT
TTAAGGGCATTTTGATTAATTTTTTATTGGTCTGCGTGTGCTAATCAAAATTGACTATAA
AATGTCAACAAAAATCAAGTTTTACAACTATTTTTCTAGTTACTCTGCCGCTCGCCTGAA
AATCAAAATCCAGACAACTTTACGAGCCGATCTAAACCCATTTCTGGATTTAGTTGTGAC
AATTGTATTAATTGTGTATATTTCTTGGTCATTTCGTTGCTTGTTTTTGTTCCTTAGATT
GAAAAAAAAAAGATTCACGTTTGTTTACCTTACTATGTAAGAATTGCAATTAAATCAAAA
ATAAATCACACCATTGAGAATTACTCTAAAAAATGTTTCTTTATTTAAACGGGTAACTTT
ATATTATCAAAAAAGGAAACAATAACTTAAGTGTATAACATGCAAATATTTAGTTTGAAC
TATAAATTCAAATAAAGCGGTTAACTAAGTTCAGTGTTGGCTAACGTGTATCGCAGCGCT
TATCGTAACGTATCGTTATCGAAttaaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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