Detailed Information [141985]
 

Strain Information
DGRC Number 141985
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL07186 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL07186 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 92B6
Related Genes Surf6 (CG4510), CG4538 (CG4538)
Received Date 11 December 2008
Original Number LL07186
Chromosome 3
Comments cn bw floating. see general information. 18Oct2012 Y.K.
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:15730335(-)
Cytological Band: 92B6
Gene Symbol-1: Surf6
CG Number-1: CG4510
FlyBase ID-1: FBgn0038746
Insertion Type-1: Intron
Gene Symbol-2: CG4538
CG Number-2: CG4538
FlyBase ID-2: FBgn0038745
Insertion Type-2: Putative Promoter
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Library & Clone Information
Strand Minus
Insertion Point 15730335
Chromosome Band 3R
Flanking Sequence 
tttacgcagactatctttctagggTTAATGAAAAGCCTTAAATCTTTAACTTTATCGTAT
TTCCTACAGATGAGGAGACCTACGAAGAGTACCTGCTGTCGGACACGGAGGAATCGGGAA
ACAAGAAAAAGAAACAACAGGGAAAACTGAAAAACCAAGACTCCGACGACGAGGATGTAG
AGGCACGCATTGCCTCCATCAAGAACAAGCTGCGCCAGAAAAAGGGACCCACAACGGAGC
GACAGCAGAAGCGACGCGAATCTAAAAAGCTTAAACGCAGCAAGGGCGTCCAGAAACTGC
TCCTCTCCTCGGCCAAAAACCTCAAAAACGAGAACGTCAAGCACCAGAAACTGAAGAATG
GCGTGGTGAAATCAGAACAGGAGACGGAGGACAGCAAGGAACAGATCCAGCCGGTCAAGG
TGCAACCGGTTTACAACCAGGAGGCCAAGATCGTCTACTCCAAGGTGGACTTTGCCGCCA
ATCCTGGAGGCAAAGCCAAGAAGTCTCACCAAAACCCCAAGGAGATTCTGAAGAAGCTGC
GCGACACCAAGAAGCACCTAACCGAACTGCGCGAACAGGGCGAAACGGACAAGGCGGCGG
AGATCCAGACGGACATCGCCTGGCGCAACGCATTCGAAttaaccattgtgggaacactag
aac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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