Detailed Information [142069]
 

Strain Information
DGRC Number 142069
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL07520 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL07520 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 69E1
Related Genes Klc (CG5433), CG10984 (CG10984)
Received Date 11 December 2008
Original Number LL07520
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:12742740(+)
Cytological Band: 69E1
Gene Symbol-1: Klc
CG Number-1: CG5433
FlyBase ID-1: FBgn0010235
Insertion Type-1: Intron
Gene Symbol-2: CG10984
CG Number-2: CG10984
FlyBase ID-2: FBgn0036305
Insertion Type-2: Putative Promoter
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Stock Request

Library & Clone Information
Strand Plus
Insertion Point 12742740
Chromosome Band 3L
Flanking Sequence 
tttacgcagactatctttctagggTTAAGAGATTGTATTTAGTATTTATCATGCTAAACG
ATTACTTAGGCCTTAGGCCTCCATTTGTGTTTACTTTGCCACACGAGCTGCCGCCGAGCA
CTTTTCCAACTAGACTTGATTATTTATGTAAAACGAATCATATTTTCCAGTAAAAAACTT
TTCTTCAAGTGGCAGTCACTTATATTTATGTGCAACATATGTACATACACAGATAAGCTC
AAGTAGATGTGTTTGTGTGTGGGTGAATGGATTTTGTCGTTCGAAttaaccattgtggga
acactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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