Detailed Information [142171]
 

Strain Information
DGRC Number 142171
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL07990 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL07990 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 70F4
Related Genes Trl (CG33261), CG33260 (CG33260)
Received Date 11 December 2008
Original Number LL07990
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:14754954(+)
Cytological Band: 70F4
Gene Symbol-1: Trl
CG Number-1: CG33261
FlyBase ID-1: FBgn0013263
Insertion Type-1: Putative Promoter
Gene Symbol-2: CG33260
CG Number-2: CG33260
FlyBase ID-2: FBgn0053260
Insertion Type-2: Putative Promoter
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Plus
Insertion Point 14754954
Chromosome Band 3L
Flanking Sequence 
CGGAATTTAGATGCATGCGTCATTTTTACTCACACTATTCTGTATCTAGGGTTAAAATGC
GGCTAGAGATGGGAGAAGTTTAGGTACCTGTTGTTCCCTTAACATACATATGTTAACCGA
TGCCAATAAGCCAGCAGCCATACTGTTAAACATAACAGAATGGTGTGGCCACCTCATTTG
TCAGCGACTGTCTGGCAACACAGCAATGCCGATAACGATTGCTTGGCAGCACAGCCTGTT
CTGTTGTTGTTTTTGGTCGGGGGCGTTGAAACTAATTCCCCGCTGTATTGTATATAAAAT
TAAACAATAGCAAAGCCATTGAGTGCTGCAATAATTTACATCACTTGCATTTACATAACG
CACTCGGCGAGCAATCGTAAATATGTGAAGACAACGAGAAACGCGTGCGAACGACAACGA
ATGGCGAAAATTGGAAGAGCATCGAAttaaccattgtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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