Detailed Information [142248]
 

Strain Information
DGRC Number 142248
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E PBac{SAstopDsRed}LL08308 / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E PBac{SAstopDsRed}LL08308 / TM6B, Tb1
Break points/Insertion Site 96F10
Related Genes CG14542 (CG14542), exo84 (CG6095)
Received Date 11 December 2008
Original Number LL08308
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3R:21878636(-)
Cytological Band: 96F10
Gene Symbol-1: CG14542
CG Number-1: CG14542
FlyBase ID-1: FBgn0039402
Insertion Type-1: 5' UTR
Gene Symbol-2: exo84
CG Number-2: CG6095
FlyBase ID-2: FBgn0039401
Insertion Type-2: Putative Promoter
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
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Library & Clone Information
Strand Minus
Insertion Point 21878636
Chromosome Band 3R
Flanking Sequence 
tttactcacactatctttctagggTTAAGGATATCTTAACTGCAAGATTTAATTACGAAA
AATGACGGAGTTTCCTAAAGATTACTCATCAACCAAAACAATAAGCGAAGGGTGTGCGCG
ATTCTAAGGTGGCCAAGACGTGTTGGCGCTTACATGCGAATAGTGCTAGTGGGAAGAAGA
AGCTTTGCCGGTGCGCTCTGAAATAAGTGTTCACACTTCAACTTAGTGAATTAGCAACAG
GTGGAAAATAATTTTAATTTATTACAATACAACACACACACATTGTAATGAATAGTATAC
ATATAGACATATGTCTTCTTGATTTTTAACGTAGATCTTTGACGAAGTCGTTTATATTTA
AACGTGAAAATGGATTTAAGAA
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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