Detailed Information [142263]
 

Strain Information
DGRC Number 142263
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL08372 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL08372 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 77C6
Related Genes CG32425 (CG32425), CG17637 (CG17637)
Received Date 11 December 2008
Original Number LL08372
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:20496607(-)(?)
Cytological Band: 77C6
Gene Symbol-1: CG32425
CG Number-1: CG32425
FlyBase ID-1: FBgn0052425
Insertion Type-1: Intron
Gene Symbol-2: CG17637
CG Number-2: CG17637
FlyBase ID-2: FBgn0037004
Insertion Type-2: Putative Promoter
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand (?)
Insertion Point 20496607
Chromosome Band 3L
Flanking Sequence 
AAGCGATCGAAAGGGATTTAAACAATTGCAAAGCGCGACAGTTTGGACGGTTTTCAGATT
GGGCTTGGCCCAAATGAGTTTGGCAGCTAAAGTCAAGTTGTCCGACTATCAAATACCCTT
TACTCAGCTATTGTAAGTGCGTTTGAGAAATTAAAAAAAAAAAAAGCGTTTGGCTTGGAA
TTTTTGGCGATTTCTGGTAGCAAGATTGGGTGTAACAATCTTGCGATGCGTCTACCTCTT
TAAAATAACCATGCCTAATAGTAACATTCTAGTT
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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