Detailed Information [142285]
 

Strain Information
DGRC Number 142285
Genotype with FlyBase Link y[*] w[*]; PBac{SAstopDsRed}LL08452 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; PBac{SAstopDsRed}LL08452 P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 76D4
Related Genes Su(z)12 (CG8013), CG7770 (CG7770)
Received Date 11 December 2008
Original Number LL08452
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: 3L:19916024(+)
Cytological Band: 76D4
Gene Symbol-1: Su(z)12
CG Number-1: CG8013
FlyBase ID-1: FBgn0020887
Insertion Type-1: CDS
Gene Symbol-2: CG7770
CG Number-2: CG7770
FlyBase ID-2: FBgn0036918
Insertion Type-2: Putative Promoter
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Genus Drosophila
Subgenus Sophophora
Species Group melanogaster
Species Subgroup melanogaster
Species melanogaster
Lethality lethal
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update 2020-04-03
Research papers using this strain
[Please submit your publication]
Stock Request

Library & Clone Information
Strand Plus
Insertion Point 19916024
Chromosome Band 3L
Flanking Sequence 
tttacgcagactatctttctagggTTAATGCCATTTGCGGCAGAACCGTCCGGATTACTG
TCTTTTTCGCGTTTTTTCGCTGGGGCCATTGTTTTTGTGTTGTTGGCAAGGGGAACGGGG
GCGGGGGCGAAGGGCGAGGGCGGTACTGACGGCGGTACGCTTTCCACGGATCACAATTAC
AACCGCACGCAATCGCTTTTAGTTGGCAAACATTTTCGCCACATCAGAGTCCATTTATAA
TCGGTTTTTGTTACTTTTCCACCGTGCAATGCGTGTTTTCTGCCGTCCTCCTTTTCGGCG
CGAAATCTTTTGCTTTTGTCTCAGCGTTATTATGGGCGCACTAGCAACGCTACGACTGCA
TGACGAATGTTTTTGTTTTACTGTTCACCAGTTGCACACTGACTAAATAATTTGTTCATA
TGCCCATTGTACTTGTTTAAGTTGATGAGTTCAAGCAGTTTATTCGCTTACAAACATTGC
AAATGAAAGCATTATTTTTTTGCGGTCATGCCAAGCATGTAAAACGCCAGTCCTAATACA
TTGCGTGTTTGCCAACACTGTTTTGATAGTCCTTCAAACAATTTGATTTCATATGCATTT
ACTTCTCCGTTTAGTACAAAACATTTTTTAAAGGTTATACTTTATATATTTTTCCTATTT
TAATTAGTAAATTGAATGAAAGACAGTAATTAGTACACCTTTCAGACAGCTGTTTTGGTC
AGTGCTGTAAAGCACGCCAGTTACAGCCATGTGGCAACGACACAATTTGCTGGTATATTT
AGTGAAAACCATATTTGGGCACACTGCACGGTTTACTCACATGCAAAAGCCGAAATTTAG
TTAAAATTATTAGCAACCGGTGAAGCAACTGTTTCATTAAACACCCTATTCCGAACAAAC
ACCTGCTGTAACTAAACCAAAATGGACAAGAAGAGCGCAGCGTTGTACAAAAGATGCAGG
CCGAGATTGAGTCGTACCAGAACCTGCAAAAATGTGAGTAAATGCCAGCCATCTGGTGGA
TAGTACCATGATTTTGTCTTATGGTGCATGGGATGTATTGTACTTTTAACTTCATGCAAT
AGCTGCTTGAGATGTGAAGCAGCGAGCAGTGCTCGGATACATGGtgggaacactagaac
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.

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