Detailed Information [140133]

Strain Information
DGRC Number 140133
Genotype with FlyBase Link y[*] w[*]; P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B PBac{SAstopDsRed}LL00701 P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype y* w*; P{w+mW.hs=FRT(whs)}2A P{ry+t7.2=neoFRT}82B PBac{SAstopDsRed}LL00701 P{y+t7.7 ry+t7.2=Car20y}96E / TM6B, Tb1
Break points/Insertion Site 85F8
Related Genes Fancl (CG12812), CG31410
Received Date 19 October 2007
Original Number LL00701
Chromosome 3
Original Source Liqun Luo, Stanford University
Original Comments
Location: ~3R:5878360(+)
Cytological Band: 85F8
Gene Symbol-1: Fancl
CG Number-1: CG12812
FlyBase ID-1: FBgn0037781
Insertion Type-1: CDS
Gene Symbol-2: CG31410
CG Number-2: CG31410
FlyBase ID-2: FBgn0051410
Insertion Type-2: Putative Promoter.
Location coordinates are based on the Drosophila Genome release 5.0.

IMPORTANT NOTES:
1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population.
2) All third chromosome flies should be yellow[1] due to the insertion at 96E.
3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1].
4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions.

Oren Schuldiner (Weizmann Institute of Science)
General Information MARCM
Reference Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
Dev. Cell (2008) 14(2) 227-38 [PubMed ID = 18267091] [RRC reference]
Last update 2015-07-16
Research papers using this strain
[Please submit your publication]
Kuo HK, McMahan S, Rota CM, Kohl KP, Sekelsky J.
Drosophila FANCM helicase prevents spontaneous mitotic crossovers generated by the MUS81 and SLX1 nucleases.
Genetics (2014) 198(3) 935-45 [PubMed ID = 25205745] [RRC reference]

Cotrufo M, Arciprete P, Fittipaldi O.
[Cardiac malformations as factors predisposing to respiratory diseases].
Arch Monaldi Mal Torace (1989) 44(1-3) 269-76 [PubMed ID = 2520574] [RRC reference]
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Library & Clone Information
Strand Plus
Insertion Point 5878360
Chromosome Band 3R
Flanking Sequence
NTNNNNNNNNCATGCGTNAGTTNAANTAACAATGATTCTNAGAGANCGTGCCTCAGTGAN
CCTTAATTATCCAATCATTATTAGTTTGGCGGGAAAAATTGAAATACGGATAACTTACCT
CACTGCCCAAAACTCCTCTGATGATGCACGCTCCACTTGGCTGAAGCTCCGCTGCCAGAC
CAGGATACTTTTGACACAGAAGGCGCTCTACATCTTCGTTACTTTCCATTATATTTGATT
TTGGTCAGATTGTGCTTGACTGACTTTTGTTTACNN
Sequence Comment Location coordinates are based on the Drosophila Genome release 5.0.


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