Kyoto Drosophila Stock Center, Kyoto Institute of Technology

Strain Information
DGRC Number	141824
Genotype with FlyBase Link	y[] w[]; PBac{SAstopDsRed}LL06553 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1]
Genotype	y^ w^; PBac{SAstopDsRed}LL06553 P{w^+mW.hs=FRT(w^hs)}2A P{ry^+t7.2=neoFRT}82B P{y^+t7.7 ry^+t7.2=Car20y}96E / TM6B, Tb¹
Break points/Insertion Site	80D3
Related Genes	CG32350, CkIIalpha (CG17520)
Received Date	19 November 2007
Original Number	LL06553
Chromosome	3
Original Source	Liqun Luo, Stanford University
Original Comments	Location: 3L:23143531(-) Cytological Band: 80D3 Gene Symbol-1: CG32350 CG Number-1: CG32350 FlyBase ID-1: FBgn0052350 Insertion Type-1: 5' UTR Gene Symbol-2: CkIIalpha CG Number-2: CG17520 FlyBase ID-2: FBgn0000258 Insertion Type-2: 3' UTR. Location coordinates are based on the Drosophila Genome release 5.0. IMPORTANT NOTES: 1) Because second chromosome was never actively selected against, a small percentage of homozygous cn bw flies resulting in white eyes can be present in the population. 2) All third chromosome flies should be yellow[1] due to the insertion at 96E. 3) A small percentage of early insertions (lower LL number) were balanced with TM3, Sb[1] instead of TM6, Tb[1]. 4) No FRT on mutator vector - thus NOT compatible with Harvard collection to create deletions. Oren Schuldiner (Weizmann Institute of Science)
General Information	MARCM
Genus	Drosophila
Subgenus	Sophophora
Species Group	melanogaster
Species Subgroup	melanogaster
Species	melanogaster
Lethality	lethal
Reference	Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L. piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning. Dev Cell (2008) 14(2) 227-38 [RRC reference]
Last update	2020-04-03
Research papers using this strain [Please submit your publication]	L?rincz P, Kenez LA, Toth S, Kiss V, Varga A, Csizmadia T, Simon-Vecsei Z, Juhasz G. Vps8 overexpression inhibits HOPS-dependent trafficking routes by outcompeting Vps41/Lt. Elife (2019) 8 [PubMed ID = 31194677] [RRC reference] Boda A, L?rincz P, Takats S, Csizmadia T, Toth S, Kovacs AL, Juhasz G. Drosophila Arl8 is a general positive regulator of lysosomal fusion events. Biochim Biophys Acta Mol Cell Res (2019) 1866(4) 533-544 [PubMed ID = 30590083] [RRC reference] Takats S, Pircs K, Nagy P, Varga A, Karpati M, Heged?s K, Kramer H, Kovacs AL, Sass M, Juhasz G. Interaction of the HOPS complex with Syntaxin 17 mediates autophagosome clearance in Drosophila. Mol Biol Cell (2014) 25(8) 1338-54 [PubMed ID = 24554766] [RRC reference]
Stock Request

Library & Clone Information

Strand	Minus
Insertion Point	23143531
Chromosome Band	3L
Flanking Sequence	tttacgcagactatctttctagggTTAATAATAGTATCATCGCATAATTATATGAAACTA TTGACAGAATGGATATAAGTGTCTTTGAATGGAAGAAAGTGGATCTTTTCAATATAATCG TCGTACCATTTGTAAAAATTCCGAACACGGCAGAAATATCGTGCTACTGCTTTCCCGAAT CAAAATCAAGCACTGAGGAAAGAAATATAAAATTAGTAATTTGTGATAAGAACAGAAATA TTCTTATTTACCTTTCAAATTGGGAGTGTATTACCTTCAAATCTCCATCCACGCGGAAAG CTATTGCTCTTTGTTCCCTTACCAGCAATAACAGCCTTGCGACTGTCACACCGGATATCA ATAATGGTATTCACATTGATATATTTGACCTTAATAGATTAACAAAAAAGCAAGCAGCTC CAATAATTGCATCTGCATATACTCAGCCTTCAAGTACGCCCTTATGCCTCAACGCAGACG TCATTGACGATAAATTGTTTGCGTTGGCGATAGGATTGGGAAATGGTGATATTCTTTTGC ATTATGGAAAAATCACAAAAAATTTTTCTGCAAATATTCGCCAACATACTGTTTCTGGAA ACGCAGTCAATGGAATACATTTTGATTTCAAAACACAACCTTTAGATACAACTCAAATAA TGTTTGTGACATGTGTTCAAAGGAGTTTATTGTTTTATGTTAAAAGAGAAATGCATAATG GATACAAATTTGTACTTGATAATGATAAAGGTAATCTTAATACCGCAGTATAATGTGCAA GCTGAGTGGCCGAAATAAATGGATCATGGTGTGTGGGACGGCAGACGCCTGTTTTATTGC CTACACCTTCC
Sequence Comment	Location coordinates are based on the Drosophila Genome release 5.0.

ID
PWD