RS lines (RS3 and RS5)

FRT-carrying insertions can be used as docking sites

RS3 and RS5 FRT-carrying insertions can be used as docking sites for FLP-mediated transgene remobilisation, using the split white+ marker strategy.

The DrosDel deletions were generated by using RS lines.

The stocks are from the Szeged Drosophila Stock Centre, Szeged University, Hungary.

>>> Stock list RS3 / RS5

Detail information will be obtained from DrosDel home page at University of Cambridge, UK.

Genotype

At present, balancers are not shown in the genotype. Some stocks have a balancer chromosome, and the others not. Please carefully examine the line before you use.
Balancers usually used are FM7h for X, SM6a for the 2nd and TM6C, Sb1 for the 3rd.

Reference

  • Edward Ryder, Fiona Blows, Michael Ashburner, Rosa Bautista-Llacer, Darin Coulson, Jenny Drummond, Jane Webster, David Gubb, Nicola Gunton, Glynnis Johnson, Cahir J. O'Kane, David Huen, Punita Sharma, Zoltan Asztalos, Heiko Baisch, Janet Schulze, Maria Kube, Kathrin Kittlaus, Gunter Reuter, Peter Maroy, Janos Szidonya, Åsa Rasmuson-Lestander, Karin Ekström, Barry Dickson, Christoph Hugentobler, Hugo Stocker, Ernst Hafen, Jean Antoine Lepesant, Gert Pflugfelder, Martin Heisenberg, Bernard Mechler, Florenci Serras, Montserrat Corominas, Stephan Schneuwly, Thomas Preat, John Roote and Steven Russell. The DrosDel collection: a set of P-element insertions for generating custom chromosomal aberrations in Drosophila melanogaster. Genetics, Vol. 167, 797-813, June 2004 doi: 10.1534/genetics.104.026658.
  • Edward Ryder, Michael Ashburner, Rosa Bautista-Llacer, Jenny Drummond, Jane Webster, Glynnis Johnson, Terri Morley, Yuk Sang Chan, Fiona Blows, Darin Coulson, Gunter Reuter, Heiko Baisch, Christian Apelt, Andreas Kauk, Thomas Rudolph, Maria Kube, Melanie Klimm, Claudia Nickel, Janos Szidonya, Peter Maróy, Margit Pal, Åsa Rasmuson-Lestander, Karin Ekström, Hugo Stocker, Christoph Hugentobler, Ernst Hafen, David Gubb, Gert Pflugfelder, Christian Dorner, Bernard Mechler, Heide Schenkel, Joachim Marhold, Florenci Serras, Montserrat Corominas, Adrià Punset, John Roote and Steven Russell. The DrosDel Deletion Collection: A Drosophila Genomewide Chromosomal Deficiency Resource. Genetics, Vol. 177, 615-629, September 2007 doi:10.1534/genetics.107.076216

Review

  • Venken and Bellen (2007) Transgenesis upgrades for Drosophila melanogaster. Development 134, 3571-3584. doi: 10.1242/dev.005686

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Last-modified: 29 Jun 2010 (Tue) 10:15:41 (2726d)